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Biochemical Analysis and Antioxidant Activity of Methanolic Leaf Extracts of Paederia foetida and Tribulus terrestris

Rojita Mishra1, Mahendra Rana2 and Satpal Singh Bisht3

1. Department of Botany, Govt. Science College Chatrapur, Odisha, India

2. Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India

3. Department of Zoology, Kumaun University, Nainital, Uttarakhand, India


Abstract: Modern science is more inclined towards natural products and research interest is increased towards the investigation of alternatives form natural sources to synthetic additives in food products and neutraceuticals. The aim of this study was to analyse chemical composition of methanolic leaf extracts of Paederia foetida and Tribulus terrestris from Ganjam, Odisha and their antioxidant activity in vitro. Total phenolic (TP), total tannins (TT) and total flavonoid (TF) contents were determined using spectrophotometric methods. Antioxidant capacity was estimated by the following methods: 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) test and Ascorbic acid equivalent assay. Moreover, a positive linear correlation was found between TP with antioxidant capacities. The plant extract of Paederia foetida have highest antioxidant activity demonstrated by far the most potent antioxidant activity, as well as TP and TT content. Our results indicate that, in addition to their traditional usage in folk medicine, these plants represent a valuable source of natural antioxidants, and thus maybe considered as great potential for the food industry, representing possible alternatives to synthetic additives. The efficiency of these herbal products can be specially targeted and enhance by employing the herbal nanotechnology tools and techniques.

Key words: Total phenolic, total tannin, total flavonoid, DPPH, antioxidant, herbal nanotechnology.
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