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Analysis of Various PGP Activities of Psychrotrophic Bacteria WBT1, and Its Efficacy under Bioformulation

Ashutosh Paliwal1, Md. Shahbaz Anwar1 and Nazia Firdous2

1. Department of Biotechnology, Kumaun University, Nainital, Uttarakhand 263136, India

2. Department of Botany (D.G. College), C.S.J.M. University, Kanpur, U.P. India


Abstract: Plant growth promoting bacteria (PGPB) is beneficial bacteria that enhance plant growth by wide multiplicity of mechanism similar to phosphate solubilisation, that help to increased in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. The methods used are isolation techniques, judgment of phosphate solubilisation and their quantification by using pikovskaya’s agar/broth, while finding of IAA production by using orthophosphoric acid and salkowski reagent their confirmation by TLC method at the same time as detection of siderophore by CAS method. Analysis of antagonistic effect against fungi by biocontrol method while shelf life and efficacy by preparing bioformalate. Isolated strain was gram negative bacteria, screening for phosphate solubilisation was done by halo zone formation in pikovskaya’s agar plate and its quantification were obtained as (201-641 μg mL-1) upon 6th days of growth. IAA production in presence of tryptophan was obtained 23.5 μg mL-1 as compare without tryptophan 16.4 μg mL-1. Biocontrol activity against pathogenic fungi was found positive, shelf life and efficacy under bioformulation were effective for the duration of 70 days and finally results of pot trail experiment designate enhancement of plant growth promotion compare to control plant. It suggests that the strain have great potential towards plant growth promoting activities and also have a best promising shelf life in normal temperature that helpful to transport the bio-formulate while a long distance. Hence, the present isolate can act as potential candidates for the development of bio-inoculants for crop plants.

Key words: PGP, shelf life, bio-formulation, psychrotroph.
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