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Phytochemical Investigation and In vitro Antifungal Activity of Essential Oil from the Leaves of Isodon Rugosus (Wall. ex Benth) Codd

Pawan Kumar1, Mohammad Suhail Javed1, Sanjay Kumar1, Anil Kumar Tiwari2 and Khazan Singh Bisht2

1. Department of Chemistry, D.S.B. Campus Kumaun University, Nainital 263002, Uttarakhand, India

2. Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India


Abstract: The essential oil from the leaves of Isodon rugosus (Wall. ex Benth) Codd growing in the Himalayan region of Jammu & Kashmir is found completely dominated by sesquiterpenoids (74.55%), with Caryophyllene <E-> (33.34%), Germacrene D (23.21%), Bicyclogermacrene (9.36%), δ-Cadinene (2.15%), α-Humulene (2.12%) and β-Elemene (1.07%) as the major compounds. Oxygenated sesquiterpenoids, monoterpenoids and oxygenated monoterpenoids constitutes the other portion of the oil. The leaves of Isodon rugosus are used in folk lore medicines in Jammu & Kashmir. The oil from the leaves showed marked antifungal activity by poisoned food technique against R. solani, however it showed moderate effect against A. tenuis, and C. graminicola. Two test strains namely F. oxysporum f.sp. ciceris and S. sclerotiorum were not affected by this oil. The mycelia growth inhibition for these fungal strains varied from 22.22%-100%. The IC50 values showed a range from 86.1 µg/mL-810 µg/mL as compared to standard fungicides with IC50 values ranging from 42.6 µg/mL-121.6 µg/mL.

Key words: Lamiaceae, isodon, antifungal, Rhizoctonia, essential oil.
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